PCR (Polymerase Chain Reaction) primer probe combination and kit for gene methylation detection of benign and malignant endometrial lesions and application of PCR primer probe combination and kit

The invention provides a PCR primer probe combination for gene methylation detection of benign and malignant endometrial lesions, a kit and application of the PCR primer probe combination and the kit, and relates to the technical field of detection of benign and malignant endometrial lesions. Comprising nucleic acid sequences as shown in 1)-4): 1) a PCR primer and a probe for CDO1 gene methylation detection; 2) a PCR primer and a probe for CELF4 gene methylation detection; 3) a PCR primer and a probe for ZNF486 gene methylation detection; 4) a PCR primer and a probe for ZSCAN12 gene methylation detection.According to the method, cervical exfoliated cells are selected as a detection sample, noninvasive detection is achieved, the acceptability of a patient can be improved, and compared with imaging and serum tumor marker detection, the method can provide higher detection sensitivity and specificity for early clinical diagnosis of endometrial cancer. 本发明提供了一种子宫内膜良恶性病变基因甲基化检测的PCR引物探针组合、试剂盒及其应用，涉及子宫内膜良恶性病变检测技术领域；包