Biomarker combination and primer probe composition for detecting oral cancer and esophageal cancer and application of biomarker combination and primer probe composition

The invention provides a biomarker combination for oral cancer and esophageal cancer detection, a primer probe composition and application of the biomarker combination and the primer probe composition, and relates to the technical field of oral cancer and esophageal cancer detection, and the biomarker combination comprises at least any two of an NID2 gene, a PAX1 gene, a ZNF154 gene and a ZNF582. According to the invention, new marker methylation is screened out as a biomarker for detecting oral cancer and esophageal cancer, and the screened new marker methylation is combined for judgment, so that the result is objective and is not interfered by human factors, and the kit has the characteristics of higher sensitivity and specificity. 本发明提供了一种用于口腔癌食管癌检测的生物标志物组合、引物探针组合物及其应用，涉及口腔癌食管癌检测技术领域，所述生物标志物组合包括NID2基因、PAX1基因、ZNF154基因和ZNF582中的任意至少两个。本发明通过筛选出新的标记物甲基化作为用于口腔癌和食管癌检测的生物标志物，并将筛选出的新的标记物甲基化组合进行判断，使其结果客观不受人为因素干扰，具有更高的灵敏度和特异度的特点。