Amplification primer group of C/EBPZ gene promoter as well as construction method and application of amplification primer group

The invention discloses an amplification primer group of a C/EBPZ gene promoter as well as a construction method and application of the amplification primer group, and belongs to the technical field of gene engineering, the C/EBPZ gene promoter is any one of A-F sequences shown in nucleotide sequences such as SEQ ID NO: 1-SEQ ID NO: 6; the amplification primer comprises an upstream primer as shown in SEQ ID NO: 7-12 and a downstream primer as shown in SEQ ID NO: 13. The six human C/EBPZ promoters can directly regulate and control the transcriptional expression of the human C/EBPZ, and the P53 and the xanthohumol inhibit the activity of the six human C/EBPZ promoters, so that the application has potential application value for revealing the transcriptional regulation and control mechanism of the human C/EBPZ and developing drugs targeting the expression level of the C/EBPZ. 本发明公开了一种C/EBPZ基因启动子的扩增引物组及构建方法和应用，属于基因工程技术领域，该C/EBPZ基因启动子为核苷酸序列如SEQ ID NO：1-SEQ ID NO：6所示的A-F序列中的任意一条；该扩增引物包含如SEQ ID NO：7-12所示上游引物和如SEQ ID