Method and equipment for tissue culture and rapid propagation of chayote
The method comprises the following steps: S1, taking a cultivated variety introduced from Vietnam as a test material, picking seed melons when the seed melons are mature, cleaning the seed melons, putting the seed melons into an incubator for accelerating germination, cutting the seed melons open wh...
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Format: | Patent |
Sprache: | chi ; eng |
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Zusammenfassung: | The method comprises the following steps: S1, taking a cultivated variety introduced from Vietnam as a test material, picking seed melons when the seed melons are mature, cleaning the seed melons, putting the seed melons into an incubator for accelerating germination, cutting the seed melons open when buds grow to about 1cm, taking out tender buds, disinfecting the tender buds with 75% alcohol for 30s under a sterile condition, and taking out the tender buds; the invention relates to the technical field of fruit seedling culture, in the rooting test stage of tissue culture seedlings, a culture box with a ventilation function is adopted as a culture container without a sterile environment, the tissue culture seedlings are inoculated into a culture medium, and the tissue culture seedlings are inoculated into the culture medium. Vermiculite with the thickness of 3-4 cm is laid in a culture box, after the vermiculite is mixed with a sugar-free carrageenan-free nutrient solution with a certain concentration, rooti |
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