Molecular marker, primer, kit and detection method for identifying authenticity of robinia pseudoacacia honey

The invention discloses a molecular marker, a primer, a kit and a detection method for identifying authenticity of robinia pseudoacacia honey, and belongs to the technical field of molecular markers. The nucleotide sequence of the molecular marker is as shown in SEQ ID NO: 1, and the primer sequences are as shown in SEQ ID NO: 2 and SEQ ID NO: 3. The primer designed by the invention has specificity and relatively high sensitivity, and detection is carried out based on DNA molecules of robinia pseudoacacia pollen existing in honey, so that theoretical support is provided, and the result is credible. Compared with a chromatography method, a stable carbon isotope method and a spectrum method, the detection method disclosed by the invention only needs to consume trace primers and a reaction system, is low in cost and has relatively low operation difficulty. 本发明公开了一种鉴定刺槐蜂蜜真伪的分子标记、引物、试剂盒及检测方法，属于分子标记技术领域。分子标记的核苷酸序列如SEQ ID NO：1所示，引物序列如SEQ ID NO：2和SEQ ID NO：3所示。本发明设计的引物具有特异性和较高的灵敏度，且本发明基于蜂蜜中存在的刺槐花粉的DNA分子进行检测，具有理论支撑，结果