Recombinant escherichia coli for synthesizing free fatty acid as well as construction method and application thereof

The invention discloses recombinant escherichia coli for synthesizing free fatty acid as well as a construction method and application of the recombinant escherichia coli. The construction method comprises the following steps: constructing a recombinant plasmid ppcnB956; the method comprises the fol...

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Hauptverfasser:	SONG HAO, HAO XUEYAN, FANG LIXIA, CAO YINGXIU
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses recombinant escherichia coli for synthesizing free fatty acid as well as a construction method and application of the recombinant escherichia coli. The construction method comprises the following steps: constructing a recombinant plasmid ppcnB956; the method comprises the following steps: by taking a recombinant plasmid ppcnB956 as a template and sg-pcnB956-F and sg-pcnB956-R as upstream and downstream primers, amplifying to obtain an sg-pcnB956 expression cassette, and seamlessly cloning the sg-pcnB956 expression cassette to a plasmid pCF to construct a recombinant plasmid pCFp; transforming the recombinant plasmid pCFp into a recombinant escherichia coli chassis MG1655 (DE3)-delta fadE for synthesizing the free fatty acid to obtain recombinant escherichia coli for synthesizing the free fatty acid; the yield of the free fatty acid synthesized by the recombinant escherichia coli strain pcnBi for synthesizing the free fatty acid can reach 2992mg/L and is increased by 300% compared with