Microorganism for treating high-concentration copper sewage based on orf133 gene and preparation method thereof

The invention provides a microorganism for treating high-concentration copper sewage based on an orf133 gene and a preparation method thereof. The microorganism is obtained by introducing the orf133 gene in a potato mitochondrial genome into escherichia coli. The Escherichia coli engineering bacterium constructed by the invention has the advantages of wide application range, capability of quickly growing at normal temperature after a delay phase, and capability of removing eutrophication pollution of a water body while treating Cu < 2 + > pollution at high environment temperature at the optimal growth temperature of 37 DEG C, and is very low in cost; the growth speed of thalli is high, so that the pollution removal period is short; and after the generated thalli are further concentrated, the absorbed Cu < 2 + > can be easily refined, and the method has the advantage of biological smelting. 本发明提供了一种基于orf133基因的处理高浓度铜污水的微生物及制备方法，该微生物由马铃薯线粒体基因组中的orf133基因导入大肠杆菌得到。本发明构建的大肠杆菌工程菌，具有适应范围广，在常温下经过延滞期后也可快速生长，37℃的最适生长温度，保