Nitrocellulose western blot membrane as well as preparation method and application thereof

The invention provides a nitrocellulose western blot membrane as well as a preparation method and application thereof, metal salts of metals with metal ions corresponding to AI-AIII and BI-BIII in the periodic table of elements and with the ion radius smaller than 1.33 A are added into a nitrocellulose membrane casting solution, and the surface of the prepared transfer printing membrane has a uniform and relatively regular circular open pore structure; compared with a traditional nitrocellulose membrane, the nitrocellulose membrane has the advantages that the overall mechanical strength is improved, the nitrocellulose membrane has good protein adsorption capacity and is particularly suitable for western blot determination and analysis, and the method is simple in process operation, relatively simple in preparation process and beneficial to industrial amplification. 本发明提供一种硝酸纤维素蛋白印迹膜及其制备方法和应用，在硝酸纤维素铸膜液中添加金属离子对应元素周期表中AI-AIII、BI-BIII且离子半径小于1.33A的金属的金属盐，制备得到的转印膜表面具备均匀且相对规则的圆形开孔结构，相对于传统的硝酸纤维素膜而言提高了整体机械强度，并具备良好的蛋白吸