Recombinant rabies virus glycoprotein antigen displayed on yeast surface, animal rabies oral vaccine and application

The invention relates to the technical field of vaccines, in particular to a recombinant rabies virus glycoprotein antigen displayed on the surface of yeast, an animal rabies oral vaccine and application. A yeast surface display technology is utilized, an extracellular antigen region of rabies virus glycoprotein is connected to a C end of saccharomyces cerevisiae Aga2 protein, GS flexible peptide is added in the middle for connection, and targeting ligand OmpH beta 1 alpha 1 spiral regions and Co1 of intestinal M cells in different combination forms are fused at the C end of the antigen region of the rabies virus glycoprotein, so that the rabies virus glycoprotein is obtained. A designed gene sequence subjected to codon optimization is inserted into a pYD1 plasmid, induced expression is performed through a saccharomyces cerevisiae EBY100 strain, and an extracellular antigen region of rabies virus glycoprotein is displayed on the surface of the saccharomyces cerevisiae EBY100 strain. The recombinant yeast subj