Method for detecting content of 4-ethyl-2, 3-dioxypiperazine formyl chloride and related substances

The invention belongs to the technical field of medicine quality detection, and particularly relates to a method for detecting the content of 4-ethyl-2, 3-dioxypiperazine formyl chloride and related substances. According to the method, aniline is adopted as a derivatization reagent, 4-ethyl-2, 3-dioxygen piperazine formyl chloride is derived, 4-ethyl-2, 3-dioxygen piperazine formyl aniline with obvious ultraviolet absorption is generated, and the method is short in derivatization time and simple and convenient to operate; according to the method, the content of 4-ethyl-2, 3-dioxypiperazine formyl chloride, known impurities and unknown impurities can be detected at the same time by using a conventional high performance liquid chromatography, combining an ultraviolet detector, taking methyl p-hydroxybenzoate as an internal standard substance and taking 4-ethyl-2, 3-dioxypiperazine formyl aniline as a reference substance and combining an aniline derivation method; according to the method, the content of 4-ethyl-