Coenzyme A preparation method based on pantothenate kinase engineering bacteria

The invention discloses a preparation method of coenzyme A based on pantothenic acid kinase engineering bacteria, and relates to the technical field of biomedicine.The preparation method of the coenzyme A comprises the following steps that S1, bacillus amyloliquefaciens is cultured, pantothenic acid...

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Hauptverfasser:	WANG WEI, PANG ZHEN, MENG XIAOCHUAN, WANG JUNYONG, CHEN LAN, YANG YANG, WANG KANGLIN, XIANG TINGTING
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses a preparation method of coenzyme A based on pantothenic acid kinase engineering bacteria, and relates to the technical field of biomedicine.The preparation method of the coenzyme A comprises the following steps that S1, bacillus amyloliquefaciens is cultured, pantothenic acid kinase genes are obtained through separation from the bacillus amyloliquefaciens, and S2, according to the pantothenic acid kinase genes obtained in the step S1, the coenzyme A is obtained. The method comprises the following steps: S1, extracting pantothenic acid kinase from a GenBank database, finding a corresponding pantothenic acid kinase nucleotide sequence from the GenBank database, and cloning the pantothenic acid kinase nucleotide sequence to an Escherichia coli expression vector pET-28a (+) to obtain a recombinant plasmid pET-28a-PANK, and S3, transferring the recombinant plasmid pET-28a-PANK obtained in the step S2 into Escherichia coli BL21 (DE3). The pantothenic acid kinase is extracted and separated fr