Preparation method and application of yeast expression triple helix structure I type recombinant human collagen

The invention discloses a preparation method and application of yeast expression triple helix structure I type recombinant human collagen, and relates to the technical field of biology. The preparation method comprises the following steps: fermenting and purifying engineering bacteria to obtain the...

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Hauptverfasser: FU SHENGWEI, FAN XIAOJU, QIAN SONG, LI XIE, LI DANLU, ZHAI YUANXIN, CHU YUN, QIAN CHENMING
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creator FU SHENGWEI
FAN XIAOJU
QIAN SONG
LI XIE
LI DANLU
ZHAI YUANXIN
CHU YUN
QIAN CHENMING
description The invention discloses a preparation method and application of yeast expression triple helix structure I type recombinant human collagen, and relates to the technical field of biology. The preparation method comprises the following steps: fermenting and purifying engineering bacteria to obtain the triple-helix structure I-type recombinant human collagen; the construction method of the engineering bacterium comprises the following steps: transforming plasmids of pMChZ-alpha1 (I), pMCrN-alpha2 (I), pPIC9K-P4H (DP)-1 and pMCeH-[alpha1 (I)] 2alpha2 (I) into host bacteria, and constructing to obtain the engineering bacterium. The I type recombinant human collagen with the triple helix structure prepared by the preparation method contains hydroxyproline, and the hydroxyproline accounts for more than 30% of total proline. Therefore, the I-type recombinant human collagen can be widely applied to the fields of cosmetics, medical cosmetology, medical instruments, biomedical materials and the like, and has good practic
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subjects BEER
BIOCHEMISTRY
CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS, ORSURGICAL ARTICLES
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
DISINFECTION, STERILISATION, OR DEODORISATION OF AIR
ENZYMOLOGY
HUMAN NECESSITIES
HYGIENE
MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS, OR SURGICALARTICLES
MEDICAL OR VETERINARY SCIENCE
METALLURGY
METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS INGENERAL
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
ORGANIC CHEMISTRY
PEPTIDES
PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
PROCESSES USING MICROORGANISMS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPECIFIC USE OF COSMETICS OR SIMILAR TOILETPREPARATIONS
SPIRITS
VINEGAR
WINE
title Preparation method and application of yeast expression triple helix structure I type recombinant human collagen
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