Preparation method and application of yeast expression triple helix structure I type recombinant human collagen
The invention discloses a preparation method and application of yeast expression triple helix structure I type recombinant human collagen, and relates to the technical field of biology. The preparation method comprises the following steps: fermenting and purifying engineering bacteria to obtain the...
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creator | FU SHENGWEI FAN XIAOJU QIAN SONG LI XIE LI DANLU ZHAI YUANXIN CHU YUN QIAN CHENMING |
description | The invention discloses a preparation method and application of yeast expression triple helix structure I type recombinant human collagen, and relates to the technical field of biology. The preparation method comprises the following steps: fermenting and purifying engineering bacteria to obtain the triple-helix structure I-type recombinant human collagen; the construction method of the engineering bacterium comprises the following steps: transforming plasmids of pMChZ-alpha1 (I), pMCrN-alpha2 (I), pPIC9K-P4H (DP)-1 and pMCeH-[alpha1 (I)] 2alpha2 (I) into host bacteria, and constructing to obtain the engineering bacterium. The I type recombinant human collagen with the triple helix structure prepared by the preparation method contains hydroxyproline, and the hydroxyproline accounts for more than 30% of total proline. Therefore, the I-type recombinant human collagen can be widely applied to the fields of cosmetics, medical cosmetology, medical instruments, biomedical materials and the like, and has good practic |
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The preparation method comprises the following steps: fermenting and purifying engineering bacteria to obtain the triple-helix structure I-type recombinant human collagen; the construction method of the engineering bacterium comprises the following steps: transforming plasmids of pMChZ-alpha1 (I), pMCrN-alpha2 (I), pPIC9K-P4H (DP)-1 and pMCeH-[alpha1 (I)] 2alpha2 (I) into host bacteria, and constructing to obtain the engineering bacterium. The I type recombinant human collagen with the triple helix structure prepared by the preparation method contains hydroxyproline, and the hydroxyproline accounts for more than 30% of total proline. 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The preparation method comprises the following steps: fermenting and purifying engineering bacteria to obtain the triple-helix structure I-type recombinant human collagen; the construction method of the engineering bacterium comprises the following steps: transforming plasmids of pMChZ-alpha1 (I), pMCrN-alpha2 (I), pPIC9K-P4H (DP)-1 and pMCeH-[alpha1 (I)] 2alpha2 (I) into host bacteria, and constructing to obtain the engineering bacterium. The I type recombinant human collagen with the triple helix structure prepared by the preparation method contains hydroxyproline, and the hydroxyproline accounts for more than 30% of total proline. 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The preparation method comprises the following steps: fermenting and purifying engineering bacteria to obtain the triple-helix structure I-type recombinant human collagen; the construction method of the engineering bacterium comprises the following steps: transforming plasmids of pMChZ-alpha1 (I), pMCrN-alpha2 (I), pPIC9K-P4H (DP)-1 and pMCeH-[alpha1 (I)] 2alpha2 (I) into host bacteria, and constructing to obtain the engineering bacterium. The I type recombinant human collagen with the triple helix structure prepared by the preparation method contains hydroxyproline, and the hydroxyproline accounts for more than 30% of total proline. Therefore, the I-type recombinant human collagen can be widely applied to the fields of cosmetics, medical cosmetology, medical instruments, biomedical materials and the like, and has good practic</abstract><oa>free_for_read</oa></addata></record> |
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subjects | BEER BIOCHEMISTRY CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS, ORSURGICAL ARTICLES CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA DISINFECTION, STERILISATION, OR DEODORISATION OF AIR ENZYMOLOGY HUMAN NECESSITIES HYGIENE MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS, OR SURGICALARTICLES MEDICAL OR VETERINARY SCIENCE METALLURGY METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS INGENERAL MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPECIFIC USE OF COSMETICS OR SIMILAR TOILETPREPARATIONS SPIRITS VINEGAR WINE |
title | Preparation method and application of yeast expression triple helix structure I type recombinant human collagen |
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