Preparation method and application of yeast expression triple helix structure I type recombinant human collagen

The invention discloses a preparation method and application of yeast expression triple helix structure I type recombinant human collagen, and relates to the technical field of biology. The preparation method comprises the following steps: fermenting and purifying engineering bacteria to obtain the triple-helix structure I-type recombinant human collagen; the construction method of the engineering bacterium comprises the following steps: transforming plasmids of pMChZ-alpha1 (I), pMCrN-alpha2 (I), pPIC9K-P4H (DP)-1 and pMCeH-[alpha1 (I)] 2alpha2 (I) into host bacteria, and constructing to obtain the engineering bacterium. The I type recombinant human collagen with the triple helix structure prepared by the preparation method contains hydroxyproline, and the hydroxyproline accounts for more than 30% of total proline. Therefore, the I-type recombinant human collagen can be widely applied to the fields of cosmetics, medical cosmetology, medical instruments, biomedical materials and the like, and has good practic