Plasmid for performing fluorescence labeling on bacterial outer membrane vesicles and preparation method thereof

The invention discloses a plasmid capable of automatically inserting a membrane localization luciferase reporter gene into a chromosome of a bacterium/recipient bacterium, the plasmid can be lost after the luciferase reporter gene is inserted into the chromosome of the bacterium/recipient bacterium, and the plasmid cannot be lost due to the fact that the plasmid can be lost. Therefore, the bacteria/recipient bacteria of which the chromosome is inserted into the luciferase reporter gene do not need to be added with antibiotics in subsequent use, so that convenience and rapidness are realized, and interference is reduced. 本发明公开一种能够自动在细菌/受体菌染色体上插入膜定位荧光素酶报告基因的质粒，该质粒将荧光素酶报告基因插入细菌/受体菌染色体后，即可质粒丢失，由于可以质粒丢失，故本发明染色体被插入荧光素酶报告基因的细菌/受体菌在后续使用中无需再加入抗生素，方便快捷且干扰减少。