Detection method for insertion mutation of self-incompatible pistil determinant CdS-RNase gene of Vietnam camellia oleifera

The invention belongs to the technical field of molecular biology and plant molecular breeding, and particularly relates to a method for detecting insertion mutation of a self-incompatible pistil determinant CdS-RNase gene of Vietnam camellia oleifera. A primer pair is designed according to the 64bp insertion mutation of the CdS-RNase gene found in camellia oleifera in Vietnam for the first time, and meanwhile, a PCR (Polymerase Chain Reaction) amplification system and conditions are optimized, so that a rapid detection method for the 64bp insertion mutation of the CdS-RNase gene is provided. The screening efficiency of the camellia tonkinensis self-incompatible pistil determinant CdS-RNase mutant can be improved, and the excavation process of the camellia tonkinensis CdS-RNase gene mutant is accelerated. 本发明属于分子生物学及植物分子育种技术领域，具体涉及一种越南油茶自交不亲和雌蕊决定因子CdS-RNase基因插入突变的检测方法。本发明根据在越南油茶中首次发现的CdS-RNase基因64bp插入突变，设计引物对，同时优化PCR扩增体系和条件，提供一种CdS-RNase基因64bp插入突变的快速检测方法。本发明可提高越南油茶自交不亲和雌蕊决定因子CdS-RNase突变体的筛选效率，加速越南油茶CdS-RNase基