Mycobacterium tuberculosis fusion protein TM and application thereof

The invention relates to a mycobacterium tuberculosis fusion protein, belongs to the field of biological medicines, and particularly discloses a novel fusion protein TM fused with mycobacterium tuberculosis antigens TB10.4 and MPT64 and application of the novel fusion protein TM. The invention provides an expression and purification method of the fusion protein, the novel fusion protein TM is used as an antigen coated elisa plate, and a mycobacterium tuberculosis specific antigen in peripheral serum is detected by preparing an ELISA detection kit. The problems that in an existing mycobacterium tuberculosis detection technology, detection operation is tedious, consumed time is long, and cost is high are solved more accurately, and therefore the mycobacterium tuberculosis detection kit has clinical application value. 本发明涉及一种结核分枝杆菌融合蛋白，属于生物医药领域，具体公开一种融合了结核分枝杆菌抗原TB10.4和MPT64新型融合蛋白TM及其应用。本发明提出了该融合蛋白的表达和纯化方法，并将此新型融合蛋白TM作为抗原包被酶标板，通过制备ELISA检测试剂盒检测外周血清中的结核分枝杆菌特异性抗原，以更加确切的解决现有结核分枝杆菌检测技术中检测操作繁琐、耗时长、费用昂贵的问题，因而具有临床应用价值。