Extraction and purification method of bose

The invention discloses a method for extracting and purifying botryine, and belongs to the technical field of enzyme catalysis production of botryine. The method comprises the following steps: (1) inactivating enzyme catalytic reaction liquid containing a glassine, and filtering through a ceramic membrane to obtain filtrate; (2) carrying out electrodialysis desalination on the filtrate to obtain a desalted solution; (3) decoloring and removing impurities from the desalted liquid through a zeolite molecular sieve column and an alumina column to obtain a decolored liquid; and (4) concentrating the decolored solution, and freeze-drying to obtain the glassine. The method disclosed by the invention is simple in process, low in cost, green and environment-friendly, high in product purity, good in discharged material quality and suitable for industrial production, and impurities such as protein, pigment and salt brought in the enzyme reaction process can be effectively removed, and toxic organic reagents are abandon