Denitrification activated sludge specific periplasmic electron transport chain activity detection method

Denitrification activated sludge specific periplasmic electron transport chain activity detection method

The invention discloses a method for detecting the activity of a specific periplasmic electron transport chain of denitrifying activated sludge, which comprises the following steps: washing the denitrifying activated sludge by using a buffer solution with the pH of 7-8 to remove an original substrat...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: DONG ZHIHUI, LU JING, ZHANG YU, JIN RENCUN
Format: Patent
Sprache:chi ; eng
Schlagworte:
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
PHYSICS
TESTING
Online-Zugang:Volltext bestellen
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page
container_issue
container_start_page
container_title
container_volume
creator DONG ZHIHUI
LU JING
ZHANG YU
JIN RENCUN
description The invention discloses a method for detecting the activity of a specific periplasmic electron transport chain of denitrifying activated sludge, which comprises the following steps: washing the denitrifying activated sludge by using a buffer solution with the pH of 7-8 to remove an original substrate, fixing the volume to the original volume of the denitrifying activated sludge by using the buffer solution with the pH of 7-8, resuspending into a centrifugal tube to obtain a resuspension, and ultrasonically crushing to extract soluble protein; uniformly mixing the soluble protein with a 50mM cytochrome c aqueous solution and a 10mM NaNO2 aqueous solution, detecting peak values at 550nm before and after the reaction, and calculating the content of the cytochrome c before and after the biological reaction; and calculating the specific periplasmic electron transport chain activity of the denitrification activated sludge according to a formula. The activity of the periplasmic electron transport chain composed of t
format Patent
fullrecord <record><control><sourceid>epo_EVB</sourceid><recordid>TN_cdi_epo_espacenet_CN118641313A</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>CN118641313A</sourcerecordid><originalsourceid>FETCH-epo_espacenet_CN118641313A3</originalsourceid><addsrcrecordid>eNqNjEEKwjAURLNxIeod4gFchIq4lVZx5cp9-SRT8yFNQvIVvL0t9ACu5sG8mbXyHSJL4YEtCaeoyQp_SOB0DW_3gq4Zdq51RuEcqI4TI8BKmXQpFGtORbT1xMuc5asdZFLmxxHik9uq1UChYrfkRu1v12d7PyCnHjWTRYT07cOY8-loGtNcmn-cH8mOQbQ</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>patent</recordtype></control><display><type>patent</type><title>Denitrification activated sludge specific periplasmic electron transport chain activity detection method</title><source>esp@cenet</source><creator>DONG ZHIHUI ; LU JING ; ZHANG YU ; JIN RENCUN</creator><creatorcontrib>DONG ZHIHUI ; LU JING ; ZHANG YU ; JIN RENCUN</creatorcontrib><description>The invention discloses a method for detecting the activity of a specific periplasmic electron transport chain of denitrifying activated sludge, which comprises the following steps: washing the denitrifying activated sludge by using a buffer solution with the pH of 7-8 to remove an original substrate, fixing the volume to the original volume of the denitrifying activated sludge by using the buffer solution with the pH of 7-8, resuspending into a centrifugal tube to obtain a resuspension, and ultrasonically crushing to extract soluble protein; uniformly mixing the soluble protein with a 50mM cytochrome c aqueous solution and a 10mM NaNO2 aqueous solution, detecting peak values at 550nm before and after the reaction, and calculating the content of the cytochrome c before and after the biological reaction; and calculating the specific periplasmic electron transport chain activity of the denitrification activated sludge according to a formula. The activity of the periplasmic electron transport chain composed of t</description><language>chi ; eng</language><subject>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES ; MEASURING ; PHYSICS ; TESTING</subject><creationdate>2024</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&amp;date=20240913&amp;DB=EPODOC&amp;CC=CN&amp;NR=118641313A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&amp;date=20240913&amp;DB=EPODOC&amp;CC=CN&amp;NR=118641313A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>DONG ZHIHUI</creatorcontrib><creatorcontrib>LU JING</creatorcontrib><creatorcontrib>ZHANG YU</creatorcontrib><creatorcontrib>JIN RENCUN</creatorcontrib><title>Denitrification activated sludge specific periplasmic electron transport chain activity detection method</title><description>The invention discloses a method for detecting the activity of a specific periplasmic electron transport chain of denitrifying activated sludge, which comprises the following steps: washing the denitrifying activated sludge by using a buffer solution with the pH of 7-8 to remove an original substrate, fixing the volume to the original volume of the denitrifying activated sludge by using the buffer solution with the pH of 7-8, resuspending into a centrifugal tube to obtain a resuspension, and ultrasonically crushing to extract soluble protein; uniformly mixing the soluble protein with a 50mM cytochrome c aqueous solution and a 10mM NaNO2 aqueous solution, detecting peak values at 550nm before and after the reaction, and calculating the content of the cytochrome c before and after the biological reaction; and calculating the specific periplasmic electron transport chain activity of the denitrification activated sludge according to a formula. The activity of the periplasmic electron transport chain composed of t</description><subject>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</subject><subject>MEASURING</subject><subject>PHYSICS</subject><subject>TESTING</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2024</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNjEEKwjAURLNxIeod4gFchIq4lVZx5cp9-SRT8yFNQvIVvL0t9ACu5sG8mbXyHSJL4YEtCaeoyQp_SOB0DW_3gq4Zdq51RuEcqI4TI8BKmXQpFGtORbT1xMuc5asdZFLmxxHik9uq1UChYrfkRu1v12d7PyCnHjWTRYT07cOY8-loGtNcmn-cH8mOQbQ</recordid><startdate>20240913</startdate><enddate>20240913</enddate><creator>DONG ZHIHUI</creator><creator>LU JING</creator><creator>ZHANG YU</creator><creator>JIN RENCUN</creator><scope>EVB</scope></search><sort><creationdate>20240913</creationdate><title>Denitrification activated sludge specific periplasmic electron transport chain activity detection method</title><author>DONG ZHIHUI ; LU JING ; ZHANG YU ; JIN RENCUN</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN118641313A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2024</creationdate><topic>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</topic><topic>MEASURING</topic><topic>PHYSICS</topic><topic>TESTING</topic><toplevel>online_resources</toplevel><creatorcontrib>DONG ZHIHUI</creatorcontrib><creatorcontrib>LU JING</creatorcontrib><creatorcontrib>ZHANG YU</creatorcontrib><creatorcontrib>JIN RENCUN</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>DONG ZHIHUI</au><au>LU JING</au><au>ZHANG YU</au><au>JIN RENCUN</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Denitrification activated sludge specific periplasmic electron transport chain activity detection method</title><date>2024-09-13</date><risdate>2024</risdate><abstract>The invention discloses a method for detecting the activity of a specific periplasmic electron transport chain of denitrifying activated sludge, which comprises the following steps: washing the denitrifying activated sludge by using a buffer solution with the pH of 7-8 to remove an original substrate, fixing the volume to the original volume of the denitrifying activated sludge by using the buffer solution with the pH of 7-8, resuspending into a centrifugal tube to obtain a resuspension, and ultrasonically crushing to extract soluble protein; uniformly mixing the soluble protein with a 50mM cytochrome c aqueous solution and a 10mM NaNO2 aqueous solution, detecting peak values at 550nm before and after the reaction, and calculating the content of the cytochrome c before and after the biological reaction; and calculating the specific periplasmic electron transport chain activity of the denitrification activated sludge according to a formula. The activity of the periplasmic electron transport chain composed of t</abstract><oa>free_for_read</oa></addata></record>
fulltext fulltext_linktorsrc
identifier
ispartof
issn
language chi ; eng
recordid cdi_epo_espacenet_CN118641313A
source esp@cenet
subjects INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
PHYSICS
TESTING
title Denitrification activated sludge specific periplasmic electron transport chain activity detection method
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-02T20%3A10%3A30IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-epo_EVB&rft_val_fmt=info:ofi/fmt:kev:mtx:patent&rft.genre=patent&rft.au=DONG%20ZHIHUI&rft.date=2024-09-13&rft_id=info:doi/&rft_dat=%3Cepo_EVB%3ECN118641313A%3C/epo_EVB%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true