Burkholderia gladioli Pseudomonas coconut poison subsp. Melastoma subsp. Gladioli microdroplet digital PCR (polymerase chain reaction) primer group, probe and application of microdroplet digital PCR primer group and probe
The invention relates to the field of biological detection, in particular to a Burkholderia gladioli microdroplet digital PCR (polymerase chain reaction) primer group, a probe and application of the Burkholderia gladioli microdroplet digital PCR primer group. The invention provides a primer group an...
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| creator | LIANG MEIDAN PENG CHENG LIANG ZHIAN CHEN YUN ZHOU WEIJIE HU LING SUN XUEQI CAO XIAFEI MAO XINWU LIN XIUMIN YIN WEILU XIAO JIAN BAI WEIBIN |
| description | The invention relates to the field of biological detection, in particular to a Burkholderia gladioli microdroplet digital PCR (polymerase chain reaction) primer group, a probe and application of the Burkholderia gladioli microdroplet digital PCR primer group. The invention provides a primer group and a probe. The primer group and the probe have nucleotide sequences as shown in SEQ ID NO: 1, SEQ ID NO: 2 and SEQ ID NO: 3. The invention provides a microdroplet digital PCR (polymerase chain reaction) quantitative detection method for burkholderia gladioli (Pseudomonas coconut poison subsp. Oryzae), which has the characteristics of strong detection specificity and high sensitivity, and is applied to the fields of food safety supervision and inspection, supportability inspection, emergency inspection of emergent food safety events and the like.
本发明涉及生物检测领域，尤其涉及唐菖蒲伯克霍尔德氏菌椰毒假单胞菌酵米面亚种微滴数字PCR引物组、探针及其应用。本发明提供了引物组和探针，其具有：如SEQ ID NO:1、SEQ ID NO:2和SEQ ID:3所示的核苷酸序列。本发明提出了一种唐菖蒲伯克霍尔德氏菌(椰毒假单胞菌酵米面亚种)微滴数字PCR定量检测方法，具有检测特异性强、灵敏度高 |
| format | Patent |
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本发明涉及生物检测领域，尤其涉及唐菖蒲伯克霍尔德氏菌椰毒假单胞菌酵米面亚种微滴数字PCR引物组、探针及其应用。本发明提供了引物组和探针，其具有：如SEQ ID NO:1、SEQ ID NO:2和SEQ ID:3所示的核苷酸序列。本发明提出了一种唐菖蒲伯克霍尔德氏菌(椰毒假单胞菌酵米面亚种)微滴数字PCR定量检测方法，具有检测特异性强、灵敏度高</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; COMPOSITIONS THEREOF ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; CULTURE MEDIA ; ENZYMOLOGY ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; PROCESSES USING MICROORGANISMS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2024</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20240913&DB=EPODOC&CC=CN&NR=118638945A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,778,883,25551,76302</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20240913&DB=EPODOC&CC=CN&NR=118638945A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>LIANG MEIDAN</creatorcontrib><creatorcontrib>PENG CHENG</creatorcontrib><creatorcontrib>LIANG ZHIAN</creatorcontrib><creatorcontrib>CHEN YUN</creatorcontrib><creatorcontrib>ZHOU WEIJIE</creatorcontrib><creatorcontrib>HU LING</creatorcontrib><creatorcontrib>SUN XUEQI</creatorcontrib><creatorcontrib>CAO XIAFEI</creatorcontrib><creatorcontrib>MAO XINWU</creatorcontrib><creatorcontrib>LIN XIUMIN</creatorcontrib><creatorcontrib>YIN WEILU</creatorcontrib><creatorcontrib>XIAO JIAN</creatorcontrib><creatorcontrib>BAI WEIBIN</creatorcontrib><title>Burkholderia gladioli Pseudomonas coconut poison subsp. Melastoma subsp. Gladioli microdroplet digital PCR (polymerase chain reaction) primer group, probe and application of microdroplet digital PCR primer group and probe</title><description>The invention relates to the field of biological detection, in particular to a Burkholderia gladioli microdroplet digital PCR (polymerase chain reaction) primer group, a probe and application of the Burkholderia gladioli microdroplet digital PCR primer group. The invention provides a primer group and a probe. The primer group and the probe have nucleotide sequences as shown in SEQ ID NO: 1, SEQ ID NO: 2 and SEQ ID NO: 3. The invention provides a microdroplet digital PCR (polymerase chain reaction) quantitative detection method for burkholderia gladioli (Pseudomonas coconut poison subsp. Oryzae), which has the characteristics of strong detection specificity and high sensitivity, and is applied to the fields of food safety supervision and inspection, supportability inspection, emergency inspection of emergent food safety events and the like.
本发明涉及生物检测领域，尤其涉及唐菖蒲伯克霍尔德氏菌椰毒假单胞菌酵米面亚种微滴数字PCR引物组、探针及其应用。本发明提供了引物组和探针，其具有：如SEQ ID NO:1、SEQ ID NO:2和SEQ ID:3所示的核苷酸序列。本发明提出了一种唐菖蒲伯克霍尔德氏菌(椰毒假单胞菌酵米面亚种)微滴数字PCR定量检测方法，具有检测特异性强、灵敏度高</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS OR TEST PAPERS THEREFOR</subject><subject>COMPOSITIONS THEREOF</subject><subject>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROCESSES OF PREPARING SUCH COMPOSITIONS</subject><subject>PROCESSES USING MICROORGANISMS</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2024</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNjL1KQ0EQhW-TQozvMHYKUQhRiaVeYmyUIPZhsju5GZy7M-zsFnnYvEtiMGAjWB3Oz3fOmt1zzV8blUiZETrByCoMC6catdeEDkGDplrAlF0TeF253cIbCXrRHk_B_MT2HLLGrCZUIHLHBQUW7Qdcmcq2p4xOEDbICTJhKKzpGizzoYEua7XRwemKAFMENBMO-D0CXf_9_Zs_gseLYTNYozhd_Oh5c_ky-2xfb8h0SW4YKFFZtu_j8fRhMn28u3-a_GezB7AabDk</recordid><startdate>20240913</startdate><enddate>20240913</enddate><creator>LIANG MEIDAN</creator><creator>PENG CHENG</creator><creator>LIANG ZHIAN</creator><creator>CHEN YUN</creator><creator>ZHOU WEIJIE</creator><creator>HU LING</creator><creator>SUN XUEQI</creator><creator>CAO XIAFEI</creator><creator>MAO XINWU</creator><creator>LIN XIUMIN</creator><creator>YIN WEILU</creator><creator>XIAO JIAN</creator><creator>BAI WEIBIN</creator><scope>EVB</scope></search><sort><creationdate>20240913</creationdate><title>Burkholderia gladioli Pseudomonas coconut poison subsp. Melastoma subsp. Gladioli microdroplet digital PCR (polymerase chain reaction) primer group, probe and application of microdroplet digital PCR primer group and probe</title><author>LIANG MEIDAN ; PENG CHENG ; LIANG ZHIAN ; CHEN YUN ; ZHOU WEIJIE ; HU LING ; SUN XUEQI ; CAO XIAFEI ; MAO XINWU ; LIN XIUMIN ; YIN WEILU ; XIAO JIAN ; BAI WEIBIN</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN118638945A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2024</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS OR TEST PAPERS THEREFOR</topic><topic>COMPOSITIONS THEREOF</topic><topic>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROCESSES OF PREPARING SUCH COMPOSITIONS</topic><topic>PROCESSES USING MICROORGANISMS</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>LIANG MEIDAN</creatorcontrib><creatorcontrib>PENG CHENG</creatorcontrib><creatorcontrib>LIANG ZHIAN</creatorcontrib><creatorcontrib>CHEN YUN</creatorcontrib><creatorcontrib>ZHOU WEIJIE</creatorcontrib><creatorcontrib>HU LING</creatorcontrib><creatorcontrib>SUN XUEQI</creatorcontrib><creatorcontrib>CAO XIAFEI</creatorcontrib><creatorcontrib>MAO XINWU</creatorcontrib><creatorcontrib>LIN XIUMIN</creatorcontrib><creatorcontrib>YIN WEILU</creatorcontrib><creatorcontrib>XIAO JIAN</creatorcontrib><creatorcontrib>BAI WEIBIN</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>LIANG MEIDAN</au><au>PENG CHENG</au><au>LIANG ZHIAN</au><au>CHEN YUN</au><au>ZHOU WEIJIE</au><au>HU LING</au><au>SUN XUEQI</au><au>CAO XIAFEI</au><au>MAO XINWU</au><au>LIN XIUMIN</au><au>YIN WEILU</au><au>XIAO JIAN</au><au>BAI WEIBIN</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Burkholderia gladioli Pseudomonas coconut poison subsp. Melastoma subsp. Gladioli microdroplet digital PCR (polymerase chain reaction) primer group, probe and application of microdroplet digital PCR primer group and probe</title><date>2024-09-13</date><risdate>2024</risdate><abstract>The invention relates to the field of biological detection, in particular to a Burkholderia gladioli microdroplet digital PCR (polymerase chain reaction) primer group, a probe and application of the Burkholderia gladioli microdroplet digital PCR primer group. The invention provides a primer group and a probe. The primer group and the probe have nucleotide sequences as shown in SEQ ID NO: 1, SEQ ID NO: 2 and SEQ ID NO: 3. The invention provides a microdroplet digital PCR (polymerase chain reaction) quantitative detection method for burkholderia gladioli (Pseudomonas coconut poison subsp. Oryzae), which has the characteristics of strong detection specificity and high sensitivity, and is applied to the fields of food safety supervision and inspection, supportability inspection, emergency inspection of emergent food safety events and the like.
本发明涉及生物检测领域，尤其涉及唐菖蒲伯克霍尔德氏菌椰毒假单胞菌酵米面亚种微滴数字PCR引物组、探针及其应用。本发明提供了引物组和探针，其具有：如SEQ ID NO:1、SEQ ID NO:2和SEQ ID:3所示的核苷酸序列。本发明提出了一种唐菖蒲伯克霍尔德氏菌(椰毒假单胞菌酵米面亚种)微滴数字PCR定量检测方法，具有检测特异性强、灵敏度高</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Burkholderia gladioli Pseudomonas coconut poison subsp. Melastoma subsp. Gladioli microdroplet digital PCR (polymerase chain reaction) primer group, probe and application of microdroplet digital PCR primer group and probe |
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