Burkholderia gladioli Pseudomonas coconut poison subsp. Melastoma subsp. Gladioli microdroplet digital PCR (polymerase chain reaction) primer group, probe and application of microdroplet digital PCR primer group and probe

The invention relates to the field of biological detection, in particular to a Burkholderia gladioli microdroplet digital PCR (polymerase chain reaction) primer group, a probe and application of the Burkholderia gladioli microdroplet digital PCR primer group. The invention provides a primer group and a probe. The primer group and the probe have nucleotide sequences as shown in SEQ ID NO: 1, SEQ ID NO: 2 and SEQ ID NO: 3. The invention provides a microdroplet digital PCR (polymerase chain reaction) quantitative detection method for burkholderia gladioli (Pseudomonas coconut poison subsp. Oryzae), which has the characteristics of strong detection specificity and high sensitivity, and is applied to the fields of food safety supervision and inspection, supportability inspection, emergency inspection of emergent food safety events and the like. 本发明涉及生物检测领域，尤其涉及唐菖蒲伯克霍尔德氏菌椰毒假单胞菌酵米面亚种微滴数字PCR引物组、探针及其应用。本发明提供了引物组和探针，其具有：如SEQ ID NO:1、SEQ ID NO:2和SEQ ID:3所示的核苷酸序列。本发明提出了一种唐菖蒲伯克霍尔德氏菌(椰毒假单胞菌酵米面亚种)微滴数字PCR定量检测方法，具有检测特异性强、灵敏度高