QPCR (quantitative polymerase chain reaction) detection method for evaluating mRNA (messenger ribonucleic acid) expression of FABP4 gene in cynomolgus monkey adipose tissue

The invention relates to the technical field of gene detection, in particular to a qPCR (quantitative polymerase chain reaction) detection method for evaluating mRNA (messenger Ribonucleic Acid) expression of FABP4 gene in cynomolgus monkey adipose tissue. The primer group provided by the invention...

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Hauptverfasser:	LONG WEIHU, ZHANG TAOPING, YANG WANJING, YANG RUJIA, YE YOUSONG, TANG DONGHONG, LI ZHELI, FAN SHENGTAO, HUANG ZHANGQIONG, JIANG QINFANG, WANG CHENYUN, LI YONGJIE
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention relates to the technical field of gene detection, in particular to a qPCR (quantitative polymerase chain reaction) detection method for evaluating mRNA (messenger Ribonucleic Acid) expression of FABP4 gene in cynomolgus monkey adipose tissue. The primer group provided by the invention can realize the specific amplification of the macaca fascicularis FABP4 gene and the reference gene GAPDH, and has no amplification signal for non-target genes in a sample; by utilizing the primer group provided by the invention, the change condition of the transcriptional level of the macaca fascicularis FABP4 gene can be relatively quantitatively detected, and the primer group is high in sensitivity, simple in method and high in repeatability. Moreover, the primer group provided by the invention is high in specificity and cannot be interfered by other genes, and an effective tool is provided for researching functions and influence factors of the machin FABP4 gene. 本发明涉及基因检测技术领域，特别是涉及一种用于评价食蟹猴脂肪组织中FABP4基因mRNA表达的qP