LightOn-AAV8-TBG-Timer system for visual targeted monitoring of oxidative stress state change of liver cells
The invention discloses a LightOn-AAV8-TBG-Timer system for visual targeted monitoring of oxidative stress state change of liver cells, the system comprises a plurality of recombinant plasmid pairs packaged by adeno-associated viruses, and any one pair of recombinant plasmids is composed of a first...
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| creator | ZHANG RUI LEI YITONG ZHANG YUNLONG FENG QIAO |
| description | The invention discloses a LightOn-AAV8-TBG-Timer system for visual targeted monitoring of oxidative stress state change of liver cells, the system comprises a plurality of recombinant plasmid pairs packaged by adeno-associated viruses, and any one pair of recombinant plasmids is composed of a first recombinant plasmid and a second recombinant plasmid; the first recombinant plasmid is pAV-IL1RN-CIBN-ST-Timer, and the second recombinant plasmid is pAV-TBG-CRY2-VP64, and the first recombinant plasmid is pAV-IL1RN-CIBN-ST-Timer; wherein the Timer protein is a tetramer mutant DsRed1-E5 and is used for monitoring oxidative stress fluorescence; the CIBN and the CRY2 form a blue light-operated starting element based on the principle that the CRY2 interacts with the chaperonin CIBN of the CRY2; the L1RN is a weak promoter; vP64 is a transcription activator; sTE represents a subcellular body targeted localization element. According to the recombinant plasmid composition constructed by the invention, the oxidative stres |
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According to the recombinant plasmid composition constructed by the invention, the oxidative stres</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PHYSICS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TESTING VINEGAR WINE |
| title | LightOn-AAV8-TBG-Timer system for visual targeted monitoring of oxidative stress state change of liver cells |
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