Protein composition for marking and capturing intermediate bridge protein and application thereof

The invention provides a protein composition for marking and capturing intermediate bridge protein and application of the protein composition, and belongs to the technical field of biology. The invention provides a protein composition for marking and capturing an intermediate bridge protein, and also provides an application of the protein composition in marking and capturing the intermediate bridge protein. The concepts of PUP-IT and split-proximity marker enzyme are combined; by adopting the method disclosed by the invention, the intermediate bridge protein of two proteins which do not interact in biology but have an upstream and downstream association relationship can be marked and captured. 本发明提供了一种标记并捕获中间桥梁蛋白的蛋白组合物及其应用，属于生物技术领域。本发明提供了一种标记并捕获中间桥梁蛋白的蛋白组合物，还提供了所述蛋白组合物在标记并捕获的中间桥梁蛋白中的应用，本发明结合了PUP-IT和split-邻近标记酶的概念，采用本发明方法能够标记并捕获在生物学上没有相互作用但存在上下游关联关系的两个蛋白的中间桥梁蛋白。