Application of drug-resistant gene CYP6AN4 of spodoptera frugiperda

The invention discloses an application of a drug-resistant gene CYP6AN4 of spodoptera frugiperda. The invention discloses a drug-resistant gene CYP6AN4 for spodoptera frugiperda, and the nucleotide sequence of the drug-resistant gene CYP6AN4 is shown as 645-2198 of SEQ ID NO.1. The cDNA sequence of the Spodoptera frugiperda CYP6AN4 gene is obtained, the Spodoptera frugiperda CYP6AN4 gene is silenced by an RNAi technology which takes effect after dsRNA and LDH are combined by a spraying method, and the mortality of the Spodoptera frugiperda CYP6AN4 gene is greatly increased by applying a pesticide after silencing; obviously, after the dsRNA of the gene is combined with LDH, the gene has important application value in the field of prevention and control of spodoptera frugiperda. 本发明公开了草地贪夜蛾抗药性基因CYP6AN4的应用。草地贪夜蛾抗药性基因CYP6AN4，核苷酸序列如SEQ ID NO.1的645-2198所示。本发明得到了草地贪夜蛾CYP6AN4基因的cDNA序列，用喷雾法使dsRNA与LDH结合后起作用的RNAi技术沉默草地贪夜蛾CYP6AN4基因，沉默后施用农药导致草地贪夜蛾死亡率大幅上升；显然该基因的dsRNA与LDH结合后在草地贪夜蛾防治领域有着重要的应用价值。