Patchouli alcohol synthase PcV1 mutant as well as coding gene and application thereof

The invention discloses a patchouli alcohol synthase PcV1 mutant as well as a coding gene and application thereof. The amino acid sequence of patchouli alcohol synthase PcV1 is as shown in SEQ ID NO. 1; according to the patchouli alcohol synthase PcV1 mutant, leucine at the 297th site of an amino acid sequence as shown in SEQ ID NO.1 is mutated into isoleucine, as shown in SEQ ID NO.2; compared with a wild expression strain PcV1, the catalytic activity of the alpha-butene of the engineering strain disclosed by the invention is obviously improved, the yield of patchouli alcohol of the PcV1 is 32.96 mg/L, and the yield of the alpha-butene is 27.04 mg/L; according to the L297I mutant, the yield of the patchouli alcohol is 1.31 mg/L, the yield of the alpha-butene is 78.28 mg/L, and conditions are created for industrial production of the alpha-butene. 本发明公开了一种广藿香醇合成酶PcV1突变体及其编码基因与应用，广藿香醇合成酶PcV1的氨基酸序列如SEQ ID NO.1所示；广藿香醇合成酶PcV1突变体为SEQ ID NO.1所示氨基酸序列第297位亮氨酸突变为异亮氨酸，如SEQ ID NO.2所示；本发明的工程菌的α-布黎烯的催化活性相比野生型表达菌株PcV1有明显提升，