Culture method for culturing super memory NK cells

The invention discloses a culture method for culturing super memory NK cells. A coating solution, an activation culture medium and an amplification culture medium are adopted in the culture method. The method comprises the following steps: (1) coating culture; (2) separating and purifying NK; (3) inoculating and culturing; (4) adding the activated culture medium and plasma; (5) adding the activated culture medium and the plasma, and adding a memory factor; (6) washing off memory factors and activating the culture medium, separating and purifying, resuspending the cells into a new culture bottle by using an amplification culture medium, culturing, adding plasma, and continuing culturing; (7) supplementing an amplification culture medium and adding plasma; (8) transferring the cell suspension in the culture bottle into a culture bag, supplementing an amplification culture medium and adding plasma; (9) supplementing an amplification culture medium and adding plasma; (10) collecting after the determination result