Saccharomyces cerevisiae engineering strain method for efficiently synthesizing (-)-alpha-bisabolol through multi-copy site integration

The invention discloses a saccharomyces cerevisiae engineering strain method for efficiently synthesizing (-)-alpha-bisabolol through multi-copy site integration, relates to the technical field of biotechnology, and solves the problem of low (-)-alpha-bisabolol synthesis efficiency. According to the...

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Bibliographische Detailangaben
Hauptverfasser: LIU JIANIE, MEI YIFAN, XU NAN, ZHANG YU, GU LINYING
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention discloses a saccharomyces cerevisiae engineering strain method for efficiently synthesizing (-)-alpha-bisabolol through multi-copy site integration, relates to the technical field of biotechnology, and solves the problem of low (-)-alpha-bisabolol synthesis efficiency. According to the technical scheme, the method for efficiently synthesizing the (-)-alpha-bisabolol through multi-copy site integration is characterized in that a saccharomyces cerevisiae engineering strain is included, a plurality of copy sites are integrated in a genome of the saccharomyces cerevisiae engineering strain, each copy site can independently express an enzyme gene related to synthesis of the (-)-alpha-bisabolol, and the enzyme gene related to synthesis of the (-)-alpha-bisabolol can be efficiently expressed through multiple copy sites. The enzyme genes include but are not limited to bisabolol synthetase, bisabolol reductase and enzyme genes related to precursor metabolism. The enzyme gene is precisely inserted into th