Nested microsphere hydrogel carrying double exosomes as well as preparation method and application of nested microsphere hydrogel

The invention is applicable to the technical field of tissue engineering, and provides a preparation method of nested microsphere hydrogel carrying double exosomes, and the preparation method comprises the following steps: preparing o-exos and exos; preparing a white porous foamed GelMA hydrogel precursor; the preparation method comprises the following steps: weighing Fibrinogen, dissolving the Fibrinogen in PBS (Phosphate Buffer Solution) containing o-exos, so as to obtain a Fibrinogen hydrogel precursor solution carrying the exosome, and carrying out photo-crosslinking polymerization, so as to obtain the Fibrinogen-exos microsphere hydrogel; the preparation method comprises the following steps: weighing a GelMA hydrogel precursor, dissolving the GelMA hydrogel precursor in PBS (Phosphate Buffer Solution) containing exos, fully and uniformly mixing the PBS containing exos with the Fibrinogeno-exos microsphere hydrogel, and carrying out photo-crosslinking polymerization, so as to obtain the GelMA exos-Fibrino