Drug-loaded bacterial outer membrane vesicle nanoparticles as well as preparation method and application thereof

The invention relates to the technical field of antibacterial drug preparation, in particular to drug-loaded bacterial outer membrane vesicle nanoparticles as well as a preparation method and application thereof. The preparation method comprises the following steps: mixing a drug-loaded nano-liposome and a bacterial outer membrane vesicle, and preparing the drug-loaded bacterial outer membrane vesicle nano-particle through a membrane extrusion method, the surface of the bacterial outer membrane vesicle expresses ClyA-antiPAO1-flag protein, and drugs loaded by the drug-loaded liposome are colistin and H89. 本发明涉及抗菌药物制备技术领域，具体涉及一种载药细菌外膜囊泡纳米颗粒及其制备方法和应用。该制备方法，包括如下步骤，将载药纳米脂质体和细菌外膜囊泡混合，通过膜挤压法制备得到所述载药细菌外膜囊泡纳米颗粒，所述细菌外膜囊泡的表面表达ClyA-antiPAO1-flag蛋白，所述载药脂质体所载药物为粘菌素和H89。