Application of aromatic retinoic acid in increasing incidence rate of barley and rice isolated microspore induced embryos

The invention provides application of aromatic retinoic acid in improving incidence rate of barley and rice isolated microspore induced embryos, and belongs to the technical field of plant cell engineering. According to the method, after the barley free microspores are pretreated by 0.15-0.25 mu M of aromatic retinoic acid, the proportion of the expanded and compact microspores is remarkably increased, and it is indicated that the barley free microspores are greatly promoted to induce and start embryonic development through aromatic retinoic acid pretreatment. 0.2-0.4 mu M of aromatic retinoic acid is added into the induction culture medium for culturing the free microspores of the rice, and the yield of the obtained calluses is also remarkably higher than that of a control group, so that the embryogenesis efficiency can be improved by adding the aromatic retinoic acid in the culture process of the free microspores of the rice. The method for increasing the incidence rate of barley/rice isolated microspore in