Method for synthesizing ethylhexylglycerin through biotransformation and special whole-cell catalyst

The invention discloses a method for synthesizing ethylhexylglycerin through biotransformation and a special whole-cell catalyst. The epoxide hydrolase mutant protein provided by the invention is any one of the following proteins: A1) the protein shown in A1) is a protein with epoxide hydrolase activity obtained by mutating at least one of 228th, 308th and 311th amino acid residues of an amino acid sequence of wild epoxide hydrolase; the protein shown in A2) is a fusion protein obtained by connecting the protein shown in A1 with a protein with a targeting function; the protein shown in A3) is a fusion protein obtained by adding a tag sequence to the tail end of the amino acid sequence of the protein shown in A1. According to the invention, site-directed mutagenesis is carried out on the active center of EHs by utilizing a molecular biological technology and a protein engineering means, and the catalytic efficiency of enzyme is successfully improved, so that the problem of low catalytic activity in the prior a