Culture medium and method for improving NK cell amplification and killing activity in serum-free environment

The invention provides a culture medium and a method for improving NK cell amplification and killing activity in a serum-free environment, and belongs to the technical field of medicine. The immune cells are NK cells, specific nutrition and stimulating factors are provided at the initial stage, the middle stage and the final stage of NK cell culture by utilizing three different culture media, namely, the culture medium A, the culture medium B and the culture medium C, and the effect of remarkably improving the amplification multiple of the NK cells and the killing activity of the NK cells on tumor cells in a serum-free environment is achieved; especially, the effect in liver cancer and lung cancer cells is excellent. The problems that a traditional serum-free culture method is low in proliferation efficiency and insufficient in cell function activity are solved, so that a safe and efficient NK cell culture scheme is provided, and a new technical support is provided for tumor immunotherapy. 本发明提供了一种用于提高无血清环境下N