Enrichment purification and application of culture solution micromolecule polypeptide group

The invention relates to the technical field of biological polypeptides, and discloses enrichment and purification and application of a culture solution small molecule polypeptide group. Transdermal peptide TAT is stably transfected to human umbilical cord-derived mesenchymal stem cells by using a genetic engineering technology, so that the human umbilical cord-derived mesenchymal stem cells continuously secrete transdermal peptide to culture solution supernatant, and the culture solution small molecule polypeptide group is obtained. The transdermal effect of the active ingredients can be improved without exogenous addition. Macromolecular proteins, impurities and the like which are difficult to penetrate through the skin in the human umbilical cord-derived mesenchymal stem cell culture solution supernatant are removed by utilizing a multi-time ultrafiltration method, TAT and active small molecule peptides are enriched, and the utilization degree of the stem cell culture supernatant in the field of skin care