Construction method of characteristic chromatogram of traditional Chinese medicine compound and method for measuring content of flavonol glycoside compounds of traditional Chinese medicine compound

The invention relates to the technical field of medicine analysis and traditional Chinese medicine detection, in particular to a construction method of a characteristic chromatogram of a traditional Chinese medicine compound composed of radix pseudostellariae, radix puerariae and herba epimedii and...

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Hauptverfasser: HAO JUAN, LI CHENGGONG, WANG FANHONG, ZAKIA
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention relates to the technical field of medicine analysis and traditional Chinese medicine detection, in particular to a construction method of a characteristic chromatogram of a traditional Chinese medicine compound composed of radix pseudostellariae, radix puerariae and herba epimedii and a method for measuring the content of flavonol glycoside compounds of the traditional Chinese medicine compound. The conditions of the liquid chromatographic analysis are as follows: gradient elution is carried out by adopting an aqueous solution of a mobile phase A acetonitrile and a mobile phase B phosphoric acid for 0-12 minutes, the volume percent of the mobile phase A is maintained to be 22% and 12-13 minutes, the volume percent of the mobile phase A is increased from 22% to 24% and 13-27 minutes, the volume percent of the mobile phase A is maintained to be 24% and 27-28 minutes, the volume percent of the mobile phase A is increased from 24% to 47% and 28-32 minutes, and the volume percent of the mobile phase