Preparation method for obtaining oligosaccharide by directional enzymolysis of polygonatum sibiricum polysaccharide

The invention discloses a preparation method for obtaining oligosaccharide through directional enzymolysis of polygonatum sibiricum polysaccharide. The preparation method comprises the following steps: (1) pretreating raw materials; 2) extracting crude polysaccharide; and 3) directional enzymolysis...

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Bibliographische Detailangaben
Hauptverfasser: LIANG KAIYUN, WU FENGHUA, LIU XINGQUAN, YU DONGHONG, WU CHANGLING, HUANG XIAOFANG, WANG PENG, WANG JIAWEI, FENG BOJIE
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention discloses a preparation method for obtaining oligosaccharide through directional enzymolysis of polygonatum sibiricum polysaccharide. The preparation method comprises the following steps: (1) pretreating raw materials; 2) extracting crude polysaccharide; and 3) directional enzymolysis of polygonatum sibiricum polysaccharide. After polygonatum kingianum crude polysaccharide is obtained through an ultrasonic-assisted enzyme method, cellulase or a compound enzyme of cellulase and pectinase is adopted to carry out directional enzymolysis reaction through an enzyme membrane coupling reactor, and then directional enzymolysis and membrane filtration combined circular reaction is carried out to obtain polygonatum kingianum oligosaccharide. The method has the advantages of high yield, low polymerization degree and uniform product distribution, overcomes the defects of low oligosaccharide extraction rate and non-uniform distribution of extracted substances in the conventional polygonatum sibiricum, and is