Nucleic acid aptamer and application thereof in preparation of product for detecting grouper iridovirus

The invention provides a nucleic acid aptamer. The nucleic acid aptamer comprises a nucleotide sequence as shown in SEQ ID NO. 1. The aptamer provided by the invention has high affinity and specificity to grouper iridovirus, and has the advantages of short preparation period, good reproducibility, s...

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Hauptverfasser: DAI SHENGSHENG, YU QING, LING FEI, CHEN JIA, QIN QIWEI, LIU MINGZHU, WANG GAOXUE, MOU RONGLI, HUANG LIN, CHENG YUAN, WEI DONGDONG, YUN YUYING, HUANG JING, LI PENGFEI
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention provides a nucleic acid aptamer. The nucleic acid aptamer comprises a nucleotide sequence as shown in SEQ ID NO. 1. The aptamer provided by the invention has high affinity and specificity to grouper iridovirus, and has the advantages of short preparation period, good reproducibility, small molecular weight, no immunogenicity, convenience in marking, convenience in in-vitro chemical synthesis, easiness in modification and substitution of different parts of the aptamer, stable sequence, easiness in transportation and storage and the like. When the aptamer is used for detecting the grouper iridovirus, the operation is simple and rapid, and higher accuracy and sensitivity can be achieved. The aptamer can be used for constructing a fluorescent molecular probe and a detection kit, and can be combined with detection equipment such as a microplate reader, a flow cytometry and a fluorescence microscope to realize accurate detection of grouper iridovirus. 本发明提供一种核酸适体,核酸适体包括如SEQ ID NO.1所示的核苷酸序列。本发明所提供的核酸适体