Universal paramagnetic particle method RNA (Ribonucleic Acid) automatic extraction kit

The invention relates to the technical field of biotechnology, in particular to a universal automatic RNA (Ribonucleic Acid) extraction kit adopting a paramagnetic particle method. The kit comprises the following components: a universal lysis solution, a binding enhancer, a magnetic bead suspension,...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: LI YINGBO, WEI ZHAO, LIU YUFANG, ZOU AILAN, GAO YUJIE, WU FAN, SUN KEFEI, LI XIAOCHEN
Format: Patent
Sprache:chi ; eng
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Beschreibung
Zusammenfassung:The invention relates to the technical field of biotechnology, in particular to a universal automatic RNA (Ribonucleic Acid) extraction kit adopting a paramagnetic particle method. The kit comprises the following components: a universal lysis solution, a binding enhancer, a magnetic bead suspension, a deproteinization solution, an eluent, a first rinsing solution and a second rinsing solution, the universal lysis solution comprises a salt component system which comprises one or more of guanidine isothiocyanate, urea and guanidine hydrochloride; the surfactant system comprises one or more of hexadecyl trimethyl ammonium bromide, polyoxyethylene mono-tert-octyl phenyl ether, sodium deoxycholate and 3-[(3-cholamidopropyl) dimethylamino] propanesulfonic acid inner salt; the compound protease system comprises one or a mixed enzyme of more of protease K, alkaline papain, amylase, cellulase, lysozyme, lywallzyme and lipase in equal weight. The RNA extracted by using the kit provided by the invention is good in integ