Non-alcoholic fatty liver cell and liver organ model and construction method thereof

The invention relates to a non-alcoholic fatty liver cell and liver organ model and a construction method thereof. According to the invention, 3beta-hydroxy-5-cholesteric acid (3beta HCA) is utilized to treat mouse primary hepatocytes or human liver organs, the non-alcoholic fatty liver cell and liver organ model is successfully constructed, the modeling method is simple, convenient and rapid, the model stability is good, in-vivo molecular and pathological states can be well simulated, and the non-alcoholic fatty liver cell and liver organ model has good application prospects. The method can be suitable for various hepatocyte types, and a reliable method is provided for establishing a high-quality in-vitro preclinical NAFLD model. 本发明涉及一种非酒精性脂肪肝细胞及肝脏类器官模型及其构建方法。本发明利用3β-羟基-5-胆甾烯酸(3βHCA)对小鼠原代肝细胞或人肝脏类器官进行处理，成功构建了非酒精性脂肪肝细胞及肝脏类器官模型，造模方法简便快捷，模型稳定性良好，能够很好地模拟体内的分子和病理状态，可适用于多种肝细胞类型，为建立高质量的体外临床前NAFLD模型提供了一种可靠的方法。