Viscosity-sensitive probe based on dicyano derivative, synthesis method and application of viscosity-sensitive probe in preparation of lipid droplet labeled product

The invention provides a viscosity-sensitive probe based on dicyano derivatives, a synthesis method and application of the viscosity-sensitive probe to preparation of lipid droplet labeling products, the probe is called LDCNs for short, the synthesis method of the probe comprises the steps that a compound 2 and p-diethylaminobenzaldehyde are dissolved in acetonitrile, piperidine is added, a reflux reaction is conducted for 4.8-5.2 h at 94-96 DEG C under the condition of N2, after the reaction is finished, the compound 2 and p-diethylaminobenzaldehyde are separated, and the viscosity-sensitive probe is obtained. Purification is carried out, such that a probe LDCNs is obtained; and the compound 2 is {2-1-[4-(dimethylamino) phenyl] ethylidene]-malononitrile}. The fluorescent probe LDCNs provided by the invention have good responsiveness to viscosity, and can be applied to fluorescence detection, lipid droplet imaging and the like as a viscosity-sensitive lipid droplet probe. 本发明提供一种基于二氰基衍生物的粘度敏感型探针、合成方法及其在制备脂滴标记