DNA methylation library construction method, DNA methylation library, DNA hybridization capture method and kit

The invention discloses a DNA methylation library construction method, a DNA methylation library, a DNA hybridization capture method and a kit. The construction method comprises the following steps: providing a test sample containing a plurality of double-stranded DNA fragments; denaturing the doubl...

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1. Verfasser:	YE BANGQUAN
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMBINATORIAL CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICOLIBRARIES MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses a DNA methylation library construction method, a DNA methylation library, a DNA hybridization capture method and a kit. The construction method comprises the following steps: providing a test sample containing a plurality of double-stranded DNA fragments; denaturing the double-stranded DNA fragment into a single-stranded DNA; carrying out ligation reaction on the single-chain DNA and the double-chain header sequence to obtain single-chain DNA linked with the double-chain header sequence; carrying out extension reaction on the single-stranded DNA connected with the double-chain header sequence to form an extension fragment, and connecting the extension fragment with the double-chain header sequence of the single-stranded DNA; the method comprises the following steps: carrying out oxidation reaction on cytosine with methylation modification in double-stranded DNA connected with a double-chain header sequence to obtain double-stranded DNA with protected cytosine; extending fragments in th