Method for determining dapagliflozin and related substances thereof by high performance liquid chromatography

The invention discloses a method for separating and determining dapagliflozin and related substances thereof by high performance liquid chromatography, which adopts an octadecyl pentafluorophenyl mixed bonded silica gel chromatographic column, takes a formic acid-water solution as a mobile phase A and methanol-acetonitrile as a mobile phase B, and performs gradient elution. The method can effectively separate dapagliflozin and related substances thereof, and is convenient to operate and short in detection time. 本发明公开了一种高效液相色谱法分离测定达格列净及其有关物质的方法，采用十八烷基五氟苯基混合键合硅胶色谱柱，以甲酸-水溶液为流动相A，甲醇-乙腈为流动B，进行梯度洗脱。本发明方法可有效分离达格列净及其有关物质，并且操作方便，检测时间短。