Snake slough (zaocys dhumnade) medicinal material, decoction piece, standard decoction and formula granule HPLC characteristic chromatogram construction method

The invention provides a snake slough (zaocys dhumnade) medicinal material, decoction piece, standard decoction and formula granule HPLC characteristic chromatogram construction method, which comprises: A) extracting a snake slough raw material by using a solvent to obtain a liquid to be detected; b) measuring the liquid to be measured by adopting a high performance liquid chromatography to obtain an HPLC characteristic chromatogram of the snake slough (zaocys dhumnade) raw material; chromatographic conditions of the high performance liquid chromatography are as follows: a chromatographic column is a C18 column; a mobile phase A is acetonitrile, a mobile phase B is 0.05% glacial acetic acid, and gradient elution is carried out. According to the method, a high performance liquid chromatography is adopted, acetonitrile-0. 05% glacial acetic acid is selected as a mobile phase for gradient elution, uric acid is taken as a reference substance, the HPLC characteristic chromatograms of snake slough (zaocys dhumnade)