Zinc porphyrin-based nanofiber material, preparation method, visual bimodal fluorescence/colorimetric ratio biosensor and application

The invention provides a zinc porphyrin-based nanofiber material, a preparation method, a visual bimodal fluorescence/colorimetric ratio biosensor and application, and belongs to the technical field of electrochemical luminescence and colorimetry. The Al-ZnTCPP-coated PAN nanofiber material is synthesized through a solvothermal method, ZnTCPP has fluorescence and colorimetric properties at the same time, after ZnTCPP is compounded with PAN, PO4 < 3-> generated by hydrolyzing AA2P through ALP can be used for attacking Al nodes, ZnTCPP is made to fall off, fluorescence/colorimetric signal changes are generated, and the Al-ZnTCPP-coated PAN nanofiber material is used for high-sensitivity sensing of ALP. And the FL signal and the colorimetric signal are subjected to ratio calculation, so that the detection limit of the material on ALP is reduced, and the sensing accuracy is improved. Meanwhile, visual bimodal ratio sensing of ALP is realized by means of mobile phone photographing and mobile phone APP color recogn