LAMP (Loop-mediated Isothermal Amplification) detection kit for detecting clostridium gangrene as well as method and application for detecting clostridium gangrene

The invention provides an LAMP (Loop-Mediated Isothermal Amplification) detection kit for detecting clostridium gangrene, a method for detecting the clostridium gangrene and application, and relates to the technical field of molecular biology. The LAMP detection primer group of the detection kit comprises an outer primer F3-1, an outer primer B3-1, an inner primer FIP-1, an inner primer BIP-1, a loop primer Loop F-1 and a loop primer Loop B-1. When the kit containing the LAMP detection primer group is used for detecting a sample infected with clostridium swollen, the kit has the characteristics of simplicity and convenience in operation, high sensitivity, good specificity and the like, and the probability of false positive and false negative can be effectively reduced; and the kit can be widely used for rapidly and accurately detecting early infections such as clostridium gangrene recessive infection, low-load infection and the like of ruminant animals. 本发明提供了一种用于气肿疽梭菌检测的LAMP检测试剂盒以及检测气肿疽梭菌的方法和应用，涉及分子生物学技术领域。所