Method for constructing intestinal epithelial cell specific knockout mouse model and application

The invention relates to the technical field of biology, in particular to a method for constructing an intestinal epithelial cell specific knockout mouse model and application. The method comprises the following steps: 1, designing a target gene sequence of a gene knockout mouse; 2, a gene of the mouse is edited through the CRISPR-Cas9 technology; 3, constructing a conditional knockout mouse to obtain a mouse model; 4, verifying the mouse model; and step 5, obtaining the cognitive effect of intestinal tract ER beta knockout on the mouse, exploring the influence of intestinal tract ER beta on the cognitive function by constructing the intestinal tract ER beta conditional knockout mouse and searching for a therapeutic target, and the method has important significance for preventing the occurrence of cognitive impairment and maintaining the cognitive function of an elderly patient. 本发明涉及生物技术领域，具体涉及一种构建肠道上皮细胞特异性敲除小鼠模型的方法及应用；步骤一：设计基因敲除小鼠的目标基因序列；步骤二：利用CRISPR-Cas9技术对小鼠的基因进行编辑；步骤三：构建条件敲除小鼠，获得小鼠模型；步骤四：验证小鼠模型；步骤五：获取肠道E