Application and method of NtSGS3a gene in regulating and controlling silencing effect of PPO7 and PPO8 genes

The invention belongs to the technical field of biology, and relates to regulation and control of a PPO gene silencing effect, in particular to application and a method of an NtSGS3a gene in regulation and control of PPO7 and PPO8 gene silencing effects. According to the test, the efficiency of mediating PPO gene silencing through a VIGS system is gradually reduced to disappear along with temperature rise, but the silence effect on PPO7 and PPO8 genes can still be kept at the high temperature of 40 DEG C by adding the vector of transient overexpression NtSGS3a for treatment, and a high-temperature-resistant system of tobacco PPO silencing based on virus induction is established. The system can still effectively silence PPO7 and PPO8 at 40 DEG C, the relative expression quantity of the genes is reduced, and the quality of tobacco leaves is effectively improved by reducing the mediated enzymatic browning reaction. A foundation is laid for a subsequent high-temperature stress test, and a related experiment thoug