Method for preparing deaminase based on aspergillus oryzae fermented traditional Chinese medicine residues
The invention provides a method for preparing deaminase based on aspergillus oryzae fermented traditional Chinese medicine residues. According to the method, traditional Chinese medicine residues, a sugar source, bean pulp powder, rice bran and ammonium sulfate are used as raw materials, aspergillus...
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Format: | Patent |
Sprache: | chi ; eng |
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Zusammenfassung: | The invention provides a method for preparing deaminase based on aspergillus oryzae fermented traditional Chinese medicine residues. According to the method, traditional Chinese medicine residues, a sugar source, bean pulp powder, rice bran and ammonium sulfate are used as raw materials, aspergillus oryzae is selected as a strain, deaminase is prepared, and the method specifically comprises the following steps that aspergillus oryzae mother bacteria are taken and placed in a solid culture medium to be subjected to activation culture and cleaned with sterile water, and aspergillus oryzae spore suspension is prepared; the preparation method comprises the following steps: dissolving 20-90 parts by mass of traditional Chinese medicine residues, 1-5 parts by mass of a sugar source, 0.5-2 parts by mass of bean pulp powder, 1-3 parts by mass of rice bran and 0.2-0.5 part by mass of ammonium sulfate in water, and cooking to obtain a fermentation culture medium; inoculating the aspergillus oryzae spore suspension into |
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