Application of RcCCD4 gene in synthesis of rosa chinensis fragrance substance dihydro-beta-ionone

The invention belongs to the technical field of plant genetic engineering, particularly relates to an application of an RcCCD4 gene in synthesis of a rosa chinensis fragrance substance, namely, dihydro-beta-ionone, and particularly provides an application of an RcCCD4 protein, the amino acid sequenc...

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Hauptverfasser: JIAN HONGYING, QIU XIANQIN, WANG QIGANG, CHEN MIN, JING WEIKUN, ZHANG HAO, FU QI, TANG KAIXUE, YAN HUIJUN, ZHANG YIXIN, WANG HUICHUN
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention belongs to the technical field of plant genetic engineering, particularly relates to an application of an RcCCD4 gene in synthesis of a rosa chinensis fragrance substance, namely, dihydro-beta-ionone, and particularly provides an application of an RcCCD4 protein, the amino acid sequence of the RcCCD4 protein is shown as SEQ ID NO: 1, and the application is the application in synthesis of the rosa chinensis fragrance substance, namely, dihydro-beta-ionone. The invention also relates to an application in cracking beta-carotene. Application in Chinese rose character improvement; the invention relates to an application in Chinese rose breeding. According to the invention, an RcCCD4 full-length sequence is cloned from Chinese ancient Chinese rose Jiuyuan; prokaryotic expression protein of RcCCD4 is obtained, and in-vitro enzyme activity analysis is completed; the function of the RcCCD4 is verified by using a VIGS technology and a gene transient overexpression technology, the function of the RcCCD4 in