Hemolytic agent for pathology and preparation method thereof

The invention relates to the technical field of pathology department, in particular to a hemolytic agent for pathology and a preparation method thereof, and the hemolytic agent comprises an anionic surfactant, polyethylene glycol and water; the anionic surfactant is selected from one or more of sodi...

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Bibliographische Detailangaben
Hauptverfasser: WANG JINGNAN, LUO FANGXIU, YU CHANGMEI, WANG BAOQIANG, DU DONGNA, LU TINGWEI
Format: Patent
Sprache:chi ; eng
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Beschreibung
Zusammenfassung:The invention relates to the technical field of pathology department, in particular to a hemolytic agent for pathology and a preparation method thereof, and the hemolytic agent comprises an anionic surfactant, polyethylene glycol and water; the anionic surfactant is selected from one or more of sodium lauryl sulfate, alkyl sodium sulfonate, alkyl aryl sodium sulfonate, sodium alkyl sulfate and secondary alkyl sodium sulfate. The invention further provides a pathological section manufacturing method which comprises the following steps: centrifuging a to-be-detected body fluid sample, and discarding supernate to obtain a precipitation solution; mixing a pathological hemolytic agent with the precipitation solution, shaking and uniformly mixing, and then centrifuging to obtain a sediment cell cluster; and removing the pathological hemolytic agent, transferring the sediment cell cluster to filter paper, then putting the sediment cell cluster into an embedding box, and carrying out conventional dehydration to obtai