Slide preparation method of canna plant root tip chromosome and application of slide preparation method

The invention provides a slide preparation method of canna plant root tip chromosomes and application of the slide preparation method, and belongs to the technical field of cell biology. The method comprises the key steps that tender root tips with the length of 0.6-1.0 cm are taken at 10-12 o'clock in the morning; performing pretreatment for 2 to 2.5 hours by using a 0.002 mol/L 8-hydroxyquinoline solution; fixing with a Carnoy's fixing solution for 20-24 hours; performing water bath dissociation on 1mol/L hydrochloric acid at 60 DEG C for 10 Soaking in distilled water for 10-15 minutes; chopping, and dyeing for 10-15 minutes by using Calibao fuchsin; and finally, vertically pressing the cover glass, carrying out microscopic examination and storing. The invention provides a stable, uniform and clear chromosome tabletting technical system with multiple division phases and high repeatability, provides technical support for ploidy identification, karyotype analysis and interspecific genetic relationship determi